![]() untargeted libraries for strain engineering.Ībstract: CRISPR editing has revolutionized plant breeding, and the MAD7 CRISPR nuclease developed by Inscripta TM is democratizing access to CRISPR technologies. Abbate will describe how to identify beneficial edits across targets involved in transcription, translation, glycosylation, secretion, protein degradation, and other cellular processes, as well as evaluate the success of using targeted vs. These libraries comprise a wide range of edit types and targets, including genome-wide and targeted knockouts, short deletions, alternate codon, transcription factor binding sites, and terminator libraries. Additionally, we will learn how multiplexed and trackable editing libraries enable the discovery of new targets. Eric Abbate, will demonstrate a novel multiplexed genome-wide editing approach to optimize the cellobiohydrolase I enzyme (CBH1) production in S. In this GEN webinar, our distinguished presenter, Dr. However, despite advances in strain engineering, optimization of protein production is still constrained by low-throughput and laborious methods that limit the success of strain optimization efforts. Generating robust production strains involves strategies that target both the protein expression cassette and the host genome. coli.Ībstract: Heterologous protein production is an indispensable tool in biotechnology and biopharma for manufacturing enzymes, protein therapeutics, and more. This work highlights the effectiveness of Inscripta’s Digital Genome Engineering platform for novel discoveries in both yeast and E. These novel conditional mutants will function as invaluable tools for future studies and industrial applications of essential genes. Initial validation experiments are proving that many of these potential hits are novel TS alleles that show distinct growth properties when comparing permissive and non-permissive growth temperatures. ![]() ![]() Following genome engineering, selection experiments have led to the identification of many potential TS alleles. During this webinar, we will showcase how we leverage the precise, trackable genome editing of the Onyx Platform to discover novel TS alleles in yeast and E. While critical for the study and use of essential genes, TS alleles are notoriously difficult to find. These conditional mutations are useful in academic settings for basic study of essential genes, and they are useful in bio-industrial settings as many essential genes are central to the production of high-value compounds. Abstract: Temperature sensitive (TS) alleles are mutations in essential genes that allow for growth at permissive temperatures and restrict or reduce growth at non-permissive temperatures.
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